In this paper, Pamela Martin and Craig Kauffman explore how Ecuador’s rights of Nature are applied in practice and the reasons why Rights of Nature are upheld in some cases and not others. Ecuador’s legal framework is used as an example, as its establishment coincides with the promotion of Rights of Nature at the global level. Because few studies focus on the implementation of Rights of Nature, the authors analyze the application of Rights of Nature in Ecuador and then proceed to an analysis of the interaction between global and local governance and the lessons learned from Ecuador’s case.